The smart Trick of flow cytometer That Nobody is Discussing
The smart Trick of flow cytometer That Nobody is Discussing
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Data-enriched characterization of mobile populations. Morphological information and facts from visuals can insert into the richness of flow cytometry details. As an example, the determine displays an usually common apoptosis assay using Annexin V and PI, including mobile imaging to characterize cells in Every population to reveal morphologically distinct features. These insights could not are attained from multiplex staining on your own.
As samples are acquired to the Attune CytPix Flow Cytometer, the high-speed brightfield digicam captures and stores images of detected events in a amount approximately six,000 pictures for every next, based on the flow amount and picture sizing. For greater adaptability, Attune Cytometric Software program enables you to regulate the picture capture frequency as needed and choose a particular gate for image capture, if essential.
(B) RAW 264.7 macrophages will often be annotated inaccurately with respect to particle count because of their complexity and noncircular borders. The volume of singlets available for Assessment improves as soon as the person trains and employs a far more accurate design.
Learn new insights into your experimental knowledge by visualizing populations using merged fluorescence and morphology facts. Our program interface wizards allow downsampling, concatenation (merged information) and dimensionality resources that happen to be developed in to the software program for an intuitive, streamlined data Investigation working experience.
"An image isn't very really worth 1000 dots, but it surely actually helps to see pictures. It is great to discover photos that correlate with staining. Such as, AnnexinV-favourable cells that happen to be morphologically diverse than balanced cells, or CD14+ monocytes that are more substantial plus more textured than CD3+/CD4+/CD14- lymphocytes.
Additionally, it can be illuminating to determine how many doublets slip by means of common singlet gates, and terrific to confirm the position of unanticipated double-favourable activities in my analysis."
Pair your Attune flow cytometer using an autosampler for greater workflow effectiveness, and incorporate robotic integration for optimum throughput.
Our researchers will design and style panels for you personally as persistently you need—totally free, no merchandise buy necessary.
The Attune NxT cytometer will be the responsible workhorse instrument your lab can rely upon for dependable and trusted efficiency. The Attune CytPix product encompasses a significant-speed brightfield digital camera and AI-driven image Examination program, with modern updates like device Understanding click here tools and an intuitive consumer interface, enabling enhanced details precision, precision, and faster insights into sample biology.
Most likely most importantly, these situations include cells of Evidently distinctive phenotypes which could lead to incorrect conclusions about double favourable occasions (specifically in unusual populations).
AI-driven automated impression Evaluation Along with the Attune Cytometric Software package interprets celebration features into unique morphology parameters that could be combined with regular fluorescence and scatter parameters to expose extremely reproducible inhabitants changes and distinctive subsets.
Application Observe: Automatic image Evaluation decreases person-to-person variability in flow cytometry gating approaches
Leverage the strength of Innovative engineering and innovation to speed up your discoveries. Rely on while in the Attune Flow Cytometer relatives to provide the dependability, velocity, and precision that set your operate apart.
No wash/no lyse leukocyte Examination with imaging. Cells were obtained from 24-hour-old blood by dilution in 1 mM EDTA ( Qualified UX/UI enables buyers to practice AI designs for expanded apps. (A) The “Teach Product” aspect presents annotation resources and real-time design schooling precision.
Gating on these extended parameters permits you to speedily and correctly determine populations of interest and ensure gating method with little if any handbook evaluate. The Attune software program offers usage of condition-of-the-art device Finding out Assessment applications within an expertly created consumer interface, which makes it extremely easy to use.
The Attune Xenith cytometer is a significant-parameter spectral analyzer, delivering equally spectral unmixing and standard flow abilities for comprehensive and adaptable cell Assessment.
Agilent Worth Promise assures you no less than ten years of instrument use from your date you purchase. If not, We are going to credit history you with the residual price of the procedure towards an upgraded product.
What seemed to be a homogeneous populace is in fact more assorted—an Perception that should be thought of when interpreting results.